Database

NYtor B.V.

Solution provided
Technology
Supplier identity
General description

NYtor is a research & development laboratory specialized in molecular diagnostics. The company's extensive expertise in qPCR ensures design of robust and sensitive assays for every specific application. These assays find worldwide application in e.g. gene expression, genotyping and detection of pathogens. Tailor-made applications and approaches are an important aspect of our service.

Type of Supplier
Biotechnology R&D Company - Diagnostics / Devices and Diagnostics Services
Sector of activity
  • High Throughput Screening
  • Molecular biology
  • Molecular Diagnostics
  • Personalized Medicine
Region
Province of Gelderland, NL
Regional access broker
  • Karien Croezen, Oost N.V. & Health Valley
Activity description
The 3 top equipments available

CFX96 Touch™ Real-Time PCR Detection System

Know-how, expertise available

NYtor has extensive experience in the design and development of multiplex qPCR assays using the molecular beacons.  Molecular beacons are single-stranded oligonucleotide hybridization probes that form a stem-and-loop structure. The loop contains a probe sequence that is complementary to a target sequence, and the stem is formed by the annealing of complementary arm sequences that are located on either side of the probe sequence. A fluorophore is covalently linked to the end of one arm and a quencher is covalently linked to the end of the other arm. Molecular beacons do not fluoresce when they are free in solution. However, when they hybridize to a nucleic acid strand containing a target sequence they undergo a conformational change that enables them to fluoresce brightly.

Molecular beacons can be used as amplicon detector probes in diagnostic assays. Because nonhybridized molecular beacons are dark, it is not necessary to isolate the probe-target hybrids to determine the number of amplicons synthesized during an assay. Molecular beacons are added to the assay mixture before carrying out gene amplification and fluorescence is measured in real time. The assay tube remains sealed. Consequently, the amplicons cannot escape to contaminate untested samples. Furthermore, the use of molecular beacons provides an additional level of specificity. Because it is very unlikely that false amplicons or primer-dimers possess target sequences for the molecular beacons, the generation of fluorescence is exclusively due to the synthesis of the intended amplicons.  Molecular beacons can be synthesized that possess differently colored fluorophores, enabling assays to be carried out that simultaneously detect different targets in the same reaction.  Molecular beacons are also extraordinarily specific. They easily discriminate target sequences that differ from one another by a single nucleotide substitution.  Therefore, molecular beacons are ideal probes for use in diagnostic assays designed for genetic screening, SNP detection, and pharmacogenetic applications.

Quality labels and accreditations

NYtor was founded in 2007 by Jacqueline Vet. It was, however, already her postdoctoral position, (1996-1998, New York) which laid the company’s foundation, as she became co-inventor and co-patent holder of the molecular beacon technology (www.molecular-beacons.org). This technology is applied in molecular assays of various in-vitro diagnostic companies like Cepheid, Biomerieux and Bio-Rad. The technology is widely used and finds application from human diagnostics to food quality testing.

Training and Staff possibilities
No
Contact information
Name
Jacqueline
Surname
Vet
Function
Managing director
Phone number
+31 (0) 24 352 88 31
Website Address
http://www.nytor.nl
Address
Toernooiveld 102
6525 EC  Nijmegen
Netherlands