The 3 top equipments available
The imPACcell platform is equipped with robots that allow a rapid and homogeneous cell seeding onto microplates, medium renewal and treatment of cells as well as labelling, automatized microscopes and an ArrayScan VTI Cellomics.
Know-how, expertise available
Cell imaging-based high content analysis (HCA) and high content screening (HCS) are essential tools in many areas of life science research and drug discovery. High content analysis employs image based cellular assays in a high throughput imaging and analysis format. This allows researchers to increase the number of questions they can ask whilst simultaneously decreasing the time taken to achieve their results. Today, we have experience using several BioApplications on the ArrayScan VTI Cellomics and take benefit from a 5-year experience on screening of bioactive molecules upon cellular targets with possible therapeutic applications. Our gaols are to increase the panel of BioApplications and the number of markers in order to reach a high robustness of screening for the main fundamental cellular functions, mainly related to proliferation, differentiation, motility, aging and death, in simple or complex culture model systems, e.g. cocultures, zebrafish.
To orient synthesis towards compounds with potent anti-metastasis effects, the platform has developed two cell models (prostate and mammary gland) for cell migration and wound healing analysis, these assays providing us a functional screen for the most promising ligands and functional information to chemists for the rational synthesis of new ligands. Beyond this project, the platform possesses today pertinent tools for analysing metastatic properties. In addition, set of assays are actually developed to quantify changes in the plasticity and motility of the cells as well as for identifying activation of target signalling pathways.
The Imaging-cell biochips core facility ImPACcell offers its skills, expertise and analytical resources in large-scale biological screening assays:
-Toxicity and activity assays for chemicals, drugs, environmental pollutants and toxins, etc.
-Specific liver toxicity assays
-Wound healing and cell motility assays
-Cell-based functional assays using fluorescent labels
-Multiplexed gene expression analysis
-Advice on cell culture and models
-High-throughput in situ imaging of organized cell systems.
Terms of availability for SMEs
Contact the Director of Federative Research Structure BIOSIT (contact below).
To help you to define the experimental process, you may contact the core facility scientific manager (firstname.lastname@example.org).
The core facility staff operates the spotting of cells and molecules, the cell cultures and the biological tests. For high-throughput tests, the image capture and analysis are carried out by the engineers, as well as data mining. Training will be provided upon request. For detailed morphological analysis, images are delivered to the project leader who treats them on his/her own.
Training and Staff possibilities