Database

Giga Zebrafish facility and transgenics

Solution provided
Technology
Supplier identity
General description

Zebrafish (Danio rerio) is a small fish whose biological characteristics make it an appropriate model for various uses in biology.
It is easy to raise and breed even in a small facility. Its high fertility, the external development of the embryos and their transparency make the observation of all developping organs possible. Larvae small size allows raising in 96 well plates.
These properties allow fast new compounds, toxicity and chemical mutations screening, and genetic studies as well.

Type of Supplier
Research InstitutionAcademic/University
Sector of activity
  • Animal models
  • Biomarkers / Biomarker Discovery
  • Cardiovascular
  • Drug Discovery / Research & Development
  • Gene Disorders
Region
Region of Wallonia, BE
Regional access broker
  • Annick Pierrard, Interface Enterprises-University of Liège
Activity description
The 3 top equipments available

-iSpawn for massive breeding and simultaneous generation of numerous embryos

-COPAS: flow cytometer for large-sized particles

-Zebrabox: for zebrafish motility and activity analysis

Know-how, expertise available

-Zebrafish housing, breeding and sanitisation

-Obtention of new transgenic lines

-Gene expression and co-expression studies

  • gene knock-down or gene overexpression

-Toxicology assays:

  • LC50
  • Teratology assays
  • Motility and activity analysis

-Zebrafish sperm cryopreservation and in vitro fecondation

-Zebrafish extracts (RNA, cDNA, genomic DNA, protein extract, fixed whole embryo)

-Zebrafish paraffin or cryostat sections

-Training to zebrafish breeding and care, egg injection or in situ hybridisation

Quality labels and accreditations

We work under L1 conditions.

All experiments must be approved by an animal welfare ethical committee.

Training and Staff possibilities
No
Training and Staff exchange terms and conditions

The staff who will work directly with lab animals must have the FELASA class B or C level certificate.

Contact information
Name
Winandy
Surname
Marie
Function
Zebrafish Facility Manager
Phone number
+32 (0) 4 366 99 71
Email address
mwinandy@ulg.ac.be
Address
GIGA - B34 - Avenue de l'Hôpital 1
4000  Liège
Belgium