Database

Centre for Protein Engineering - Protein Factory

Solution provided
Technology
Supplier identity
General description

The Centre for Protein Engineering at the University of Liege is a multidisciplinary centre covering a large number of domains from molecular to structural biology. It presents a solid experience in genetic engineering, bacteriology, protein production and purification ("Protein Factory"), enzymology, fast kinetics, surface plasmon resonance (SPR), microcalorimetry, optical spectroscopies (e.g. circular dichroism), fluorescence imaging microscopy, X-ray crystallography, molecular modelling.

Type of Supplier
Research Institution
Sector of activity
  • Bioinformatics
  • Biomanufacturing
  • High Throughput Screening
  • Microbiology
  • Molecular biology
Region
Region of Wallonia, BE
Regional access broker
  • Annick Pierrard, Interface Enterprises-University of Liège
Activity description
The 3 top equipments available

 

  • BioFlo 5000 (New Brunswick) Sterilisable-in-Place 80L Fermentor for bacterial and yeast Fermentation

  • AKTA explorer 10S Air (GE Healthcare) with FRAC 950 and 96-well autosampler adapted for 2D-LS protein chromatography

  • Microlab Star Liquid Handling Workstation(Hamilton Robotics) with 8 x 1000µL Independent Channels, one 96-Probe Head, and one Microlab Swap with 140 Rack Runner for High-Throughput Mutagenesis and High-Throughput Screening

Terms of availability for SMEs

The Centre of Protein Engineering at the University of Liege has developed an integrated recombinant protein expression and purification platform (i.e. “Protein Factory”), which is very active at producing proteins of interest for both the academic and industrial communities and offers state-of-the-art technology and equipment. Protein Factory encompasses genetic engineering, high throughput mutagenesis and cloning in a variety organisms and cell lines (bacterial strains, yeasts), analytical or pilot scale recombinant protein production (shaking incubators and 1L to 60L fermentors for batch and fed-batch cultures), cell harvesting or supernatant cleaning using continuous centrifugation or hollow fiber filtration, cell disintegration to recover proteins produced in the intracellular compartment and protein purification at the analytical and pilot scales. Furthermore, we are currently developing a high throughput mutagenesis platform, based on a Microlab Star Hamilton automated liquid handling workstation.

Training and Staff possibilities
No
Contact information
Name
Alain
Surname
Brans
Function
Senior scientist
Phone number
+32 (0) 4 366 34 50
Email address
abrans@ulg.ac.be
Address
B6 - Allee de la Chimie 3
4000  Liège
Belgium